Multi-Angle Light Scattering Detectors



Multi-Angle Static Light Scattering is the preferred analytical technique for determining absolute molar masses, sizes (radius of gyration Rg, also known as root mean square radius RMS) and conformation of all types of macromolecules and particles in solution such as proteins, biopolymers, polymers and particles, liposomes, micelles, encapsulated proteins and many more. MALS provides absolute measurements because they are made without reference to molar mass standards, column calibration, or molecular conformation.

 



DAWN® HELEOS II:

  • 18 angles
  • 120 mW Laser
  • Molar Mass Range: ? 103 to > 109 g/mole (Daltons)
  • Size Range (Radius): 10 nm to > 500 nm
  • Absolute calibration with toluene
  • Learn more



miniDAWN™ TREOS:

  • 3 angles
  • 60 mW Laser
  • Molar Mass Range: ? 103 to > 106 g/mole (Daltons)
  • Size Range (Radius): 10 nm to > 50 nm
  • Absolute calibration with toluene
  • Learn more

 

 






MALS measurements work by calculating the amount of scattered light at each angle detected. This process overcomes the problems associated with low angle light scattering detectors (typically there is around ten times the noise at an angle of 11° or below compared to 90°) and allows a reliable and accurate measure of the light scattered. The amount of light scattered is then related to the molar mass. MALS also determines the size of the molecule, because angular dependence of the scattered light is measured. Here, the size measurement is known as the root mean square (rms) radius also known as "radius of gyration".
It is important to know that the plurality of detectors at different angles improves the accuracy and the precision of the results. However, it is not only the number of angles that determines the quality of the measurements, but rather the engineering of the instrument, too. Superior signal-to-noise ratios and sophisticated algorithms for data management are unique features of the Wyatt instrument and software design, which have been advanced and refined since more than 30 years.
For a full explanation of light scattering theory, please see our theory section.


 

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