Combining SEC/GPC with MALS and DLS
Size Exlusion Chromatography (SEC), also known as Gel Permeation Chromatography (GPC), has been widely used to characterize the molar mass and aggregation state of proteins as well as the molar mass distribution of biopolymers and polymers. However, SEC in itself is a relative method, relying on calibration with well known standards.
Both MALS and DLS can be readily coupled to an SEC system to provide the absolute molar mass, root-mean square radius, and hydrodynamic radius of individual peaks of interest. In addition, SEC-MALS can be used for the assessment of size heterogeneity (aggregation and fragmentation), molar mass distribution, degree of pegylation or glycosylation of proteins, stoichiometry of complexes, and conformational information.
Just by adding a Wyatt Technology MALS detector to your existing chromatographic system you will be determining absolute molar masses and sizes without ever having to calibrate you GPC again!
Each Wyatt DAWN® and miniDAWN® MALS detector comes with ASTRA® 6, the most powerful GPC/SEC software package for collecting and analyzing data associated with protein, macromolecule, polymer and nanoparticle characterization. This versatile software package is used for data collection and analysis of either batch (unfractionated) or chromatographically separated samples.
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