Understanding Field-Flow-Fractionation Theory
Field-Flow-Fractionation (FFF) is a one-phase chromatography technique invented by Professor Calvin Giddings in 1966. The Flow-FFF separation mechanism works based on hydrodynamic forces taking place in a separation channel. The smaller particles are transported faster along the channel than the larger particles and are eluting before the larger ones.
Flow-FFF solves the most difficult separation, fractionation and sizing tasks in the macromolecular and colloidal arenas. The advantage of Flow-FFF over traditional SEC column chromatography is the ability to separate both soluable and colloidal components over a wide size range as well as sensitive and "sticky" samples (no stationary phase, no interaction). Its resolution is similar to sedimentation equilibrium, but it surpasses even this benchmark-sizing tool when one considers its flexibility, speed, and ease of use.
Flow-FFF is invaluable and indispensable in key areas of research and development in molecular biology, nanotechnology, and environmental analyses, including the separation and characterization of proteins and aggregates, liposomes, emulsions, viral particles, polysaccharides, nanoparticles, polymer latex particles, colloidal soil suspensions, humic substances, chemical mechanical polishing slurries (CMP), and many more.
Wyatt Technology's Eclipse® Flow-FFF separation system went to market in 2001 and has made an immediate impact in popularizing Flow-FFF. The fully automated, software controlled Eclipse® system uses only one pump to generate detector flow, cross flow and injection flow. This innovative concept to generate all three flow streams from one main flow has been a breakthrough for the Flow-FFF method.
Flow-FFF Separation using Wyatt Technology's Eclipse® AF4:
Copyright © 2014 Wyatt Technology Europe GmbH